1. STAINING
Immerse the gel in a 0,1 % Coomassie brillant blue G-250 (50 % methanol solution for 1 hour. When preparing the Coomassie Blue reagent is advisable to f ilter the solution before using .
In some applications, the dye can be reused. However, for protein identification purpose by mass spectrometry is preferable to prepare a fresh solution.
2. DESTAINING
Immerse the gel in a 40 % methanol solution. Replace the solution several times to wash out the unbounded dye.
The time required by the spots or bands to develop the colour depends on the gel size and its thickness. Gel destaining can be performed overnight if you reduce methanol concentration.
After observing the colour in bands or spots it is recommended to discard the staining solution and leave the gel in Milli-Q water for 24 hours .
3. CONSERVATION:
In Milli-Q water at room temperature.
